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Proficiency Testing/EQA / 2025 Surveys / Genetics and Molecular Pathology /
Cytogenetics

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CAP/ACMG FISH, CONST/HEMAT DISORDERS - CYF
CYF
Analytes/procedures in bold type are regulated for proficiency testing by the Centers for Medicare & Medicaid Services (CMS).

Disease/Procedure Challenges per Shipment Number of Shipments
A B
Constitutional and Hematologic Disorders Two shipments per year
FISH for constitutional disorder - slides 1 1
FISH for constitutional disorder - dry challenge 2 2
FISH for hematologic disorder - slides 1 1
FISH for hematologic disorder - dry challenge 2 2

Additional Information

  • CYF 2025-A:
    Constitutional disorder (two slides) - SRY
    Hematologic disorder (two slides) - 20q del*1

  • CYF 2025-B:
    Constitutional disorder (two slides) - (HIRA (TUPLE1))
    Hematologic disorder (two slides) - (CBFB*2)
    1For this challenge, participants should use the probe set used to interrogate deletion of 20q12 in their laboratories.
    2For this challenge, participants should use the probe set used to interrogate CBFB rearrangements in their laboratories.

  • CYF is prepared from cell suspension samples. For FISH in paraffin-embedded tissues, see programs CYH, CYJ, CYK, CYL, and CYALK.
  • This program is only for laboratories that perform both hybridization and interpretation under the same CLIA number.

Program Information

  • Four slides and four dry challenges
  • For recommendations and requirements, see flow chart

Shipping Schedule

  • Shipment A: April 7
  • Shipment B: August 25






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